a new sperm agglutinin factor from marine snail telescopium telescopium: an evaluation with goat (capra hircus) cauda epididymal spermatozoa

نویسندگان

samata maji

uttam datta

manik lal hembram

چکیده

background: lectins the multivalent carbohydrate binding proteins are also employed to ascertain differentiation and characterization of the specific cell surface carbohydrate ligands/ receptors of different cell types including spermatozoa and posses novel biomedical importance. objective: sf50 the sialic acid specific lectin was employed on goat cauda epididymal spermatozoa to investigate its effect(s) on the physiology of sperm cells if any. materials and methods: a protein factor sf50 was obtained from spermatheca/ ovotestis gland of marine snail telescopium telescopium by precipitation with 50% ammonium sulfate. different concentrations of sf50 and bovine serum albumin (bsa) as control were added with washed goat cauda epididymal spermatozoa and observed immediately and after 20 s and 60 s of incubation. results: sf50 treated sperm cells exhibited head-to-head type agglutination. the degree of agglutination varied (p

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عنوان ژورنال:
international journal of reproductive biomedicine

جلد ۸، شماره ۱، صفحات ۱۰-۰

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